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Biodegradation of Poly(ß-hydroxybutyrate) by a novel isolate of Streptomyces bangladeshensis 77T-4

類別:期刊論文

學年 / 學期:100-2

出版日期:2012-07-01 00:00:00

著者:Ke-Jen Hsu; Min Tseng; Don, Trong-Ming; Mei-Kwei Yang

單位:淡江大學化學工程與材料工程學系

出版者:Taipei: Academia Sinica * Institute of Plant and Microbial Biology

著錄名稱、卷期、頁數:Botanical Studies 53(3), pp.307-313

摘要:A thermophilic Streptomyces bangladeshensis isolate designated 77T-4 that can degrade poly(β-hydroxybutyrate) (PHB) was isolated from a soil sample in Taiwan. Isolate 77T-4 grew well in ureafructose oatmeal medium and produced a clear zone around the colony when grown on an agar plate containing emulsified PHB, indicating the presence of an extracellular PHB depolymerases. A PHB-degrading enzyme was purified to homogeneity from the culture supernatant. The molecular weight of the PHB-degrading enzyme was estimated to be approximately 40 kDa. A small portion of the N-terminal sequence (Ala-Val-Pro-Leu-Thr-Arg ) of the purified enzyme was determined and found to share a significant homology with that of the PHB depolymerase of Streptomyces sp. MG.

語言:en_US

ISSN:1817-406X

期刊性質:國外

收錄於:SCI

通訊作者:Mei-Kwei Yang

審稿制度:

國別:TWN

出版型式:電子版