出版日期：2019-04-01 00:00:00

著者：Yau-Hung Chen; Hung-Yin Tai; Earl Fu; Trong-Ming Don

著錄名稱、卷期、頁數：International Journal of Biological macromolecules 126, p.159–169

摘要：To fulfill the properties of membrane for guided bone tissue regeneration, chitosan (CS) and calcium phosphates were blended to produce porous hybrid membranes by lyophilization. We synthesized three different calcium phosphates: calcium deficient hydroxyapatite (CDHA), biphasic calcium phosphate (BCP) and β‑tricalcium phosphate (TCP) by a reverse emulsion method followed by calcination, and compared their efficacy on bone regeneration. The CDHA/CS, BCP/CS, and TCP/CS membranes had an interconnected pore structure with porosity of 91–95% and pore size of 102–147 μm. These hybrid membranes could promote the permeability and adhesiveness to bone cells as demonstrated by in-vitro cell culture of primary osteoblast. Particularly, the CDHA/CS and BCP/CS could further increase the cell attachment and differentiation, whereas the BCP/CS and TCP/CS could enhance cell proliferation. Finally, these hybrid membranes were assessed for guided bone regeneration in the critical-size calvarial bone defects created in SD rats. Histological and histomorphometric analyses revealed that the BCP/CS membrane had the most effective bone regeneration compared to the other two hybrid membranes. At three-week post-surgery, the BCP/CS membrane could enhance new bone generation up to 57% of the original bone defect area. The BCP/CS membrane thus has the potential to be applied for guided bone

關鍵字：Bone regeneration;Chitosan;Calcium phosphate

語言：en_US

期刊性質：國外

收錄於：SCI EI

通訊作者：Trong-Ming Don

審稿制度：是

國別：NLD

出版型式：電子版,紙本